Sunday, July 26, 2020

Enviromental Sampling of Volatile Organic Compounds


adafruit, bee, bee keeping, gnuplot, sparkfun, volatile organic compounds, cloake board, olfactory fatigue, reproducibility

adafruit, bee, bee keeping, gnuplot, sparkfun, volatile organic compounds, cloake board, olfactory fatigue, reproducibility
During past and this summer, I have been able to smell the backyard hives, but the human nose is not designed to quantify or map a smell - see this link about Olfactory Fatigue.    Here's how I compared the smell of 3 hives and compared each hive smell to background using a Volatile Organic Compound (VOC) sensor.

I purchased 2 breakout boards by adafruit, one measures VOC concentration and the other measures temperature and humidity. Seen in the photos:
  • two breakout boards plugged into a mini stick-on breadboard
  • red colored sparkfun arduino microprocessor attached to an upside down white plastic storage lid using black nylon standoffs by adafruit
  • a mini serial cable connecting the microprocessor and laptop
  • laptop attached to the ladder's paint-tray using a bungee cord
 adafruit, bee, bee keeping, gnuplot, sparkfun, volatile organic compounds, cloake board, olfactory fatigue, reproducibility
My first backyard environmental measurement of VOC was meant as a dry-run, but luckily I did not need to modify my experiment design:
  • chalk marked or flagged a 6 x 4 grid surrounding 3 hives - 4 grid points are skipped due to large shrubs
  • selected a day with no wind so as not to disperse the VOC which pool near the hives
  • selected an evening to avoid when most flowering plants produce VOC
  • warmed-up the sensor system outdoors for an hour before sampling
  • setup at a grid point, and sampled for 60 seconds. I stepped away from the 6 x 4 grid during sampling as my body both produces and disperses VOC
  • after sampling 20 grid points, I repeated the first 5 grid points to examine reproducibility
A few words of explanation regarding the map of VOC surrounding 3 backyard hives:
  • VOC are shown in parts per billion (ppb) units.  As I do not have access to a reference gas mixture, you can think of these VOC values as relative values.
  • The central (middle) hive had the highest concentration of VOC.  This hive is a massive stack of boxes with 2 queens separated by a cloake board.   On the bottom of this stack is an over wintered hive.   On the top of this stack is a medium size swarm catch (March 23, 2020).
  • A VOC plume from the central hive merged with the hive shown at the bottom of the map.  The hive at the bottom of the map is a large overwintered hive.
  • The hive at the top of the map is inconsequential in VOC production and resembles a background value.  This hive is a tiny swarm catch (March 13, 2020).

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